The BioXp system, along with custom-built consumable BioXp kits, automates the synthesis of mRNA, streamlining the overall workflow and expediting the time to product to just days instead of weeks or months. The available kits for mRNA synthesis enable flexibility in input so researchers can select a 1-step or 2-step workflow for mRNA synthesis.
| Steps | BioXp De novo mRNA synthesis kit | BioXp Select mRNA synthesis kit |
|---|---|---|
| Input | The BioXp De novo mRNA synthesis kit, combined with the BioXp system, provides the first push-button, walk-away end-to-end automation solution for the production of synthetic mRNA from DNA sequence. | The BioXp Select mRNA synthesis kit enables the synthesis of mRNA from an input DNA template. |
| Workflow | It automates the entire workflow including the complex and time-consuming process of synthesizing the DNA template for the generation of the final synthetic mRNA product. | When combined with the BioXp DNA cloning kit it provides a rapid, 2-step automated workflow for synthesis of synthetic mRNA. |
| Output | Purified, capped and tailed synthetic mRNA | Purified, capped and tailed synthetic mRNA |
| Runtime | ~ 18 hours | ~ 4-6 hours depending on length |
Product format: Single-stranded capped and tailed mRNA, and linear double-stranded DNA fragments
Features: 5’ ARCA or CleanCap® AG (3’OMe); poly(A) tail (median 150 As)
Nucleoside modification options:
Output size: 0.4–1.8 kb per mRNA
Order throughput: 1 to 16 samples per run
Configuration: 8 and 16 reactions per run
Yield: Minimum 5 µg per mRNA for CleanCap® AG (3’OMe) modified mRNA and minimum 1 µg per mRNA for 5’ ARCA modified mRNA
Total turnaround time: 10 to 12 days
Classification: Research use only
Product format: Contains reagents necessary to synthesize at least 50 micrograms of capped and tailed synthetic mRNA using plasmid or PCR purified DNA template.
Features: 5’ CleanCap® AG (3’OMe) capping
Nucleoside modification options:
Output size: 0.4- 10kb per mRNA
Order throughput: 1 to 48 reactions per run
Configuration: 8, 16, 24 and 48 reactions per run
Yield: Minimum yield 50 µg mRNA per well
Classification: Research use only